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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
Rabbit Anti Thr286 Pcamkiiα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Santa Cruz Biotechnology anti-(t286)pcamkiiα antibody
Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Cell Signaling Technology Inc mouse anti pcamkii monoclonal
Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of <t>pCaMKII</t> in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.
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Image Search Results


Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of pCaMKII in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.

Journal: Cell reports

Article Title: Rational designing of oscillatory rhythmicity for memory rescue in plasticity-impaired learning networks.

doi: 10.1016/j.celrep.2022.110678

Figure Lengend Snippet: Figure 2. Physiological relevance of NMDAR/AMPAR signaling during learning and memory (A) Schematic illustration of immunohistochemistry (IHC) or western blot analysis (WB) following contextual fear conditioning (CFC) training. (B and C) Representative images (B) and summary data (C) of pCaMKII in pyramidal neurons (enclosed by parallel dashed lines) and PV-positive neurons in the CA1 region of wild-type (WT) and PV-KO mice after CFC training or naive (home cage) conditioning; n = 45–64 cells from three mice/group. Scale bar: 50 mm. (D) Western blot analysis showing that CFC training increases pGluR1 levels in the hippocampus of both WT and PV-KO mice; n = 3 mice/group. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (one-way ANOVA followed by Tukey’s test). See also Figure S4.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-pCaMKII (Thr-286) Cell Signaling Technology Cat#12716; RRID: AB_271388 Mouse anti-PV Swant Cat#235; RRID: AB_10000343 Rabbit anti-pGluR1 (S831) Millipore Cat#AB5847; RRID: AB_11211981 Mouse anti-GluR1 Millipore Cat#MAB2263; RRID: AB_11212678 Rabbit anti-b-actin Cell Signaling Technology Cat#4970; RRID: AB_2223172 Bacterial and virus strains AAV2/9-hSyn-DIO-mCherry-P2A-HAHsgCaMKII-WT Taitool Bioscience AAV2/9-WY2498 AAV2/9-hEF1a-DIO-hChR2(H134R)-mCherry Taitool Bioscience AAV2/9-S0170-9 AAV2/9-hEF1a-DIO-mCherry Taitool Bioscience AAV2/9-S0197 Chemicals, peptides, and recombinant proteins Paraformaldehyde MP Biomedicals Cat#150146 DAPI Fluoromount-G Yeasen Biotechnology Cat#36308ES20 Protease inhibitor cocktail Roche Cat#04693132001 Critical commercial assays BCA protein assay Thermo Fisher Scientific Cat#23227 Enhanced chemiluminescence (ECL) detection system Yeasen Biotechnology Cat#36222 Experimental models: Organisms/strains Mouse: gCaMKIILoxP/LoxP Lab of Eric N. Olson N/A Mouse: PV-Cre The Jackson Laboratory JAX: 008069; RRID: IMSR_JAX:008069 Software and algorithms ANY-maze automated tracking system Stoelting https://www.stoeltingco.com/ any-maze-video-tracking-software-1224.html ImageJ NIH https://imagej.nih.gov/ij/ Prism Graphpad https://www.graphpad-prism.cn NeuroExplorer 5 Nex Technologies https://www.neuroexplorer.com/ MATLAB MathWorks https://www.mathworks.com/products/matlab/ Statistical Toolbox MathWorks https://www.mathworks.com/products/ statistics.html AccuSleep Github https://github.com/zekebarger/AccuSleep gcc complier GNU project https://gcc.gnu.org/ MT19937 Yukihiro Matsumoto http://www.math.keio.ac.jp/ matumoto/emt.html Code archived This paper https://doi.org/10.5281/zenodo.6357778 Other Fiber optic cannula Inper N/A

Techniques: Immunohistochemistry, Western Blot